Coran A G, Drongowski R A, Wesley J R
Int Surg. 1984 Apr-Jun;69(2):107-12.
Many shock studies utilize unconditioned random source animals. To determine whether unconditioned random source dogs and colony bred dogs differ in response to septic shock, the following experiment was undertaken. Colony bred puppies and unconditioned random source puppies were pretreated with methylprednisolone and were subject to live E. coli induced septic shock. Resuscitation was carried out with Ringer's lactate and 5% albumin. Cardiac output dropped to less than 50% of control values in all animals. The colony bred puppies maintained a significantly higher cardiac output and pulse pressure and required a longer time for their cardiac output to decrease to 50% of control levels than did the unconditioned animals. In addition, the serum acid phosphatase rose significantly (indicating cellular damage) in the unconditioned group and did not change in the colony bred dogs. Marked hyperglycemia occurred in the unconditioned puppies whereas only a mild decrease in serum glucose was seen in the colony bred animals. These data indicate that extreme caution should be exercised in interpreting the physiological response of the unconditioned animal to experimental septic shock.
许多休克研究使用未经条件化的随机来源动物。为了确定未经条件化的随机来源犬和群体饲养犬对脓毒性休克的反应是否存在差异，进行了以下实验。群体饲养的幼犬和未经条件化的随机来源幼犬用甲基强的松龙进行预处理，然后接受活大肠杆菌诱导的脓毒性休克。用乳酸林格氏液和5%白蛋白进行复苏。所有动物的心输出量均降至对照值的50%以下。与未经条件化的动物相比，群体饲养的幼犬维持着显著更高的心输出量和脉压，并且其心输出量降至对照水平的50%所需的时间更长。此外，未经条件化组的血清酸性磷酸酶显著升高（表明细胞损伤），而群体饲养犬的血清酸性磷酸酶没有变化。未经条件化的幼犬出现明显的高血糖，而群体饲养动物的血清葡萄糖仅轻度降低。这些数据表明，在解释未经条件化动物对实验性脓毒性休克的生理反应时应极其谨慎。
