Ikeda H, Inoue M, Omura S
J Antibiot (Tokyo). 1983 Mar;36(3):283-8. doi: 10.7164/antibiotics.36.283.
Three macrolide antibiotic-producing strains, the spiramycin producer Streptomyces ambofaciens, the tylosin producer Streptomyces fradiae, and the cirramycin producer Streptomyces cirratus easily formed protoplasts when treated with lysozyme in hypertonic medium. Each type of protoplast was regenerated to a mycelial form at a frequency of 90 to 100% in regeneration medium supplemented with a plasma expander. In the spiramycin producer S. ambofaciens and the tylosin producer S. fradiae, antibiotic productivities of the regenerated progeny were drastically changed. Some regenerated progeny from the three strains gave higher production. One from S. ambofaciens showed about twice the productivity of the original strain and one obtained after two rounds of protoplast regeneration from S. fradiae produced about three times as much as the original strain.
三种产生大环内酯类抗生素的菌株，即产生螺旋霉素的波赛链霉菌、产生泰乐菌素的弗氏链霉菌和产生卷霉素的卷曲链霉菌，在高渗培养基中用溶菌酶处理时很容易形成原生质体。在添加血浆扩容剂的再生培养基中，每种类型的原生质体都能以90%至100%的频率再生为菌丝体形式。在产生螺旋霉素的波赛链霉菌和产生泰乐菌素的弗氏链霉菌中，再生后代的抗生素生产力发生了巨大变化。这三种菌株的一些再生后代产量更高。波赛链霉菌的一个再生后代的生产力约为原始菌株的两倍，弗氏链霉菌经过两轮原生质体再生后获得的一个后代的产量约为原始菌株的三倍。
