液相色谱法同时测定食品中的硫胺素和核黄素
Simultaneous determination of thiamine and riboflavin in foods by liquid chromatography.
作者信息
Augustin J
出版信息
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):1012-5.
PMID:6501141
Abstract
Recent methods for determination of thiamine (thiochrome) and riboflavin by liquid chromatography (LC) are outlined and discussed, and a new method allowing the simultaneous determination of these 2 vitamins by using a single fluorescence detector is described. This system involves an ODS 5 micron ultrasphere column and a pH 7.5 mobile phase composed of 0.005M tetrabutyl ammonium phosphate in methanol-water (20 + 80).
摘要
概述并讨论了通过液相色谱法（LC）测定硫胺素（硫色素）和核黄素的最新方法，并描述了一种使用单个荧光检测器同时测定这两种维生素的新方法。该系统包括一个5微米的ODS超球柱和一种pH 7.5的流动相，该流动相由甲醇-水（20 + 80）中的0.005M磷酸四丁铵组成。
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