Habib A A, Omar A A, Sarg T M
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):939-41.
Santonin gives a characteristic alkaline vs acidic difference spectrum. This was used for its estimation in pharmaceuticals and in the crude drug. Santonin was first extracted and purified through a specific partition procedure; then the difference absorbance was measured either at the maximum, 285 nm, or the minimum, 242 nm. The percentage of santonin can be calculated either by reference to the difference absorbance of a reference santonin sample, treated similarly, or by making use of the determined absorptivity. Measurement at the maximum is advisable, especially when the crude drug is assayed, because natural contaminants may interfere with the difference absorbance at the minimum.
山道年呈现出典型的碱性与酸性差异光谱。这被用于其在药物制剂和生药中的含量测定。首先通过特定的分配程序提取并纯化山道年；然后在最大吸收波长285nm或最小吸收波长242nm处测量差异吸光度。山道年的含量百分比既可以通过参照经过类似处理的参比山道年样品的差异吸光度来计算，也可以利用已测定的吸光系数来计算。建议在最大吸收波长处进行测量，尤其是在测定生药时，因为天然杂质可能会干扰最小吸收波长处的差异吸光度。
