Sakipova Zuriyadda, Wong Nikki Siu Hai, Bekezhanova Tolkyn, Shukirbekova Alma, Boylan Fabio
Kazakh National Medical University, Almaty, Republic of Kazakhstan.
School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.
PLoS One. 2017 Mar 16;12(3):e0173714. doi: 10.1371/journal.pone.0173714. eCollection 2017.
Santonin, a powerful anthelmintic drug that was formely used to treat worms, is Artemisia cina's main constituent. However, due to its toxicity to humans, it is no longer in use. Kazakhstan is looking to introduce this plant as an anthelmintic drug for veterinary purposes, despite the known toxic properties of the santonin. The objective of this study was to develop a fast and specific method for the identification of santonin and its precise quantitation using HPLC-UV in order to avoid unnecessary intoxication, which is paramount for the development of veterinary medicines. The results obtained showed that santonin appears at around 5.7 minutes in this very reliable HPLC method. The validation of the method was performed by the investigation of parameters such as precision, accuracy, reproducibility and recovery. The method was used to identify and quantify santonin in leaves of A. scoparia, A. foetida, A. gmelinni, A. schrenkiana, A. frigida, A. sublesingiana, A terra-albae, and A. absinthium from Kazakhstan as well as in three different extracts of leaves of A. cina. This study has provided a faster and simpler method for the identification and quantification of this compound in other species of Artemisia of economic importance.
山道年是蛔蒿的主要成分，它曾是一种用于治疗蠕虫的强效驱虫药。然而，由于其对人体有毒性，已不再使用。尽管已知山道年具有毒性，但哈萨克斯坦仍希望将这种植物作为兽药引入。本研究的目的是开发一种快速、特异的方法，利用高效液相色谱-紫外检测法（HPLC-UV）鉴定山道年并对其进行精确定量，以避免不必要的中毒，这对兽药开发至关重要。所得结果表明，在这种非常可靠的HPLC方法中，山道年在约5.7分钟出现。通过对精密度、准确度、重现性和回收率等参数的研究对该方法进行了验证。该方法用于鉴定和定量哈萨克斯坦的滨蒿、恶臭蒿、准噶尔蒿、伊犁蒿、冷蒿、中亚苦蒿、白叶蒿和洋艾叶片中的山道年，以及蛔蒿叶片的三种不同提取物中的山道年。本研究为鉴定和定量其他具有经济重要性的蒿属植物中的该化合物提供了一种更快、更简便的方法。
