Vanderslice J T, Brownlee S R, Cortissoz M E
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):999-1007.
Chromatographic analysis for vitamin B-6 in complex samples imposes certain requirements on the analyst, who must extract completely the bound, unstable vitamers without loss, remove interfering compounds, and provide clean extracts for analysis. The analyst also has to contend with the problems inherent in all methods, such as sample collection, storage, preparation, and homogenization. However, chromatography provides a means of identifying and quantitating all forms of the vitamin, and thus provides the possibility of addressing the problem of the bioavailability of specific vitamers. It also allows automation, which is absolutely essential in coping with the large numbers of samples that are generated in areas such as quality control. These factors are all addressed here, and chromatographic results for various meat and other food products are presented to illustrate the variations in vitamin content that occur from sample to sample, the agreement with microbiological results, and that liquid chromatography (LC) has come of age in dealing with complex biological samples, such as food and food products.
对复杂样品中的维生素B-6进行色谱分析，对分析人员提出了一定要求，分析人员必须完全提取出结合态、不稳定的维生素异构体且不造成损失，去除干扰化合物，并提供用于分析的纯净提取物。分析人员还必须应对所有方法中固有的问题，如样品采集、储存、制备和匀浆。然而，色谱法提供了一种识别和定量维生素所有形式的方法，从而为解决特定维生素异构体的生物利用度问题提供了可能性。它还允许自动化操作，这在处理质量控制等领域产生的大量样品时绝对至关重要。本文将阐述所有这些因素，并给出各种肉类和其他食品的色谱分析结果，以说明不同样品间维生素含量的差异、与微生物学结果的一致性，以及液相色谱法在处理食品和食品产品等复杂生物样品方面已经成熟。
