通过氯仿简单、快速且定量地释放周质蛋白。
Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
作者信息
Ames G F, Prody C, Kustu S
出版信息
J Bacteriol. 1984 Dec;160(3):1181-3. doi: 10.1128/jb.160.3.1181-1183.1984.
Abstract
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.
摘要
我们介绍了一种方法，通过用氯仿处理细胞，可以快速、简单且定量地释放周质蛋白。所有测试的氨基酸结合蛋白在氯仿处理过程中都保持了其活性。这种方法使对大量菌株的周质蛋白成分进行分析成为可能。
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