Bauza M T, Smith R V, Knutson D E, Witter F R
J Chromatogr. 1984 Sep 14;310(1):61-9. doi: 10.1016/0378-4347(84)80068-7.
A method has been developed for determination of the xanthine drug, pentoxifylline, and three of its metabolites (a secondary alcohol and two carboxylic acids) in human milk. The method requires pre-extraction with hexane to remove lipids followed by extraction with dichloromethane or dichloromethane-isopropanol (4:1). Absolute extraction recoveries were between 76-90%. Pentoxifylline and its alcohol metabolite (as the trifluoroacetate) and the carboxylic acid metabolites (as ethyl esters) were measured in separate gas chromatographic steps using a nitrogen detector. Determinations of pentoxifylline and its three metabolites were 96-99% accurate and standard deviations of 5-10% were observed for samples at or above the lower practical sensitivity limit (10 ng/ml) for the assay. Pentoxifylline and its metabolites were stable in breast milk for three weeks when stored at -15 degrees C.
已开发出一种方法，用于测定母乳中的黄嘌呤药物己酮可可碱及其三种代谢物（一种仲醇和两种羧酸）。该方法需要先用己烷进行预萃取以去除脂质，然后用二氯甲烷或二氯甲烷 - 异丙醇（4:1）进行萃取。绝对萃取回收率在76%至90%之间。己酮可可碱及其醇代谢物（作为三氟乙酸盐）和羧酸代谢物（作为乙酯）在单独的气相色谱步骤中使用氮检测器进行测定。己酮可可碱及其三种代谢物的测定准确度为96%至99%，对于该测定方法，在等于或高于较低实际灵敏度极限（10 ng/ml）的样品中观察到5%至10%的标准偏差。己酮可可碱及其代谢物在-15℃下储存时，在母乳中可稳定三周。
