Lambert W E, Yousouf M A, Van Liedekerke B M, De Roose J E, De Leenheer A P
Laboratoria voor Medische Biochemie en voor Klinische Analyse, Gent, Belgium.
Clin Chem. 1989 Feb;35(2):298-301.
We describe a sensitive and specific liquid-chromatographic assay for pentoxifylline and three of its metabolites in human plasma and urine. Addition of hydrochloric acid to the sample before extraction, and incorporation of acetic acid in the chromatographic eluent, allow the simultaneous determination of the four compounds plus an internal standard in one chromatographic run. Unlike gas-chromatographic procedures, this method does not involve derivatization no similar analysis of serum or plasma samples has been described before now. The method has been applied successfully to routine analysis and to pharmacokinetic studies.
我们描述了一种灵敏且特异的液相色谱法，用于测定人血浆和尿液中的己酮可可碱及其三种代谢物。在萃取前向样品中加入盐酸，并在色谱洗脱液中加入乙酸，可在一次色谱运行中同时测定这四种化合物及一种内标物。与气相色谱法不同，该方法无需衍生化，且此前尚无类似的血清或血浆样品分析方法被报道。该方法已成功应用于常规分析和药代动力学研究。
