Chou P P, Bailey J L
Clin Chem. 1986 Jun;32(6):1026-8.
We developed and evaluated a simple liquid-chromatographic method for urinary pentachlorophenol (PCIP) determination with ultraviolet detection. PCIP and the internal standard (2,3,5,6-tetrachlorophenol, TCP) were extracted with a solid-phase extraction column, and 50 microL of the collected eluate was injected into the chromatograph. The intra-assay and interassay CVs were 6.6% (n = 20) and 7.9% (n = 10), respectively. The analytical recoveries varied from 89% to 96%, with absolute recoveries ranging from 82% to 88%. Thirty urine specimens collected from 30 unexposed individuals were analyzed by the current method, and results were all less than the minimum detection limit, 0.25 mg/L. Results for five specimens from workers who had been exposed to PCIP ranged from 7.0 to 12.4 mg/L. The standard curve was linear to 300 mg/L. This procedure is adaptable to automated analysis, and therefore suitable for monitoring industrial exposure of workers.
我们开发并评估了一种采用紫外检测的简单液相色谱法来测定尿中五氯苯酚（PCIP）。PCIP和内标物（2,3,5,6 - 四氯苯酚，TCP）用固相萃取柱进行萃取，收集50微升洗脱液注入色谱仪。批内和批间变异系数分别为6.6%（n = 20）和7.9%（n = 10）。分析回收率在89%至96%之间，绝对回收率在82%至88%之间。用本方法对30名未接触者采集的30份尿液标本进行分析，结果均低于最低检测限0.25毫克/升。对5名曾接触PCIP的工人的标本检测结果在7.0至12.4毫克/升之间。标准曲线在300毫克/升范围内呈线性。本方法适用于自动化分析，因此适合监测工人的职业接触情况。
