Belshe R B, Van Voris L P, Bartram J, Crookshanks F K
J Infect Dis. 1984 Dec;150(6):834-40. doi: 10.1093/infdis/150.6.834.
One hundred three young children were inoculated intranasally with either influenza A/California/10/78 cold-recombinant vaccine (10(6.7) 50% tissue culture infective doses [TCID50] per child), CR-37 (H1N1), or influenza A/Washington/897/80 cold-recombinant vaccine (10(6.5) TCID50 per child), CR-48 (H3N2). The vaccine was well tolerated. Of the 51 children vaccinated with CR-37 (H1N1), 45 were initially seronegative for this virus; 33 of the 45 became infected with the vaccine virus, as indicated by a fourfold rise in antibody titer or by shedding of vaccine virus. Of the 52 children vaccinated with CR-48 (H3N2), six were initially seronegative and all were infected; 46 were initially seropositive and 25 of these children developed fourfold rises in antibody titer. An outbreak of influenza A (H3N2 type predominated) occurred in Huntington one to three months after the children were vaccinated. Significantly fewer febrile illnesses occurred in the CR-48 (H3N2) vaccine group than among the CR-37 (H1N1) vaccine group. Hemagglutination inhibition antibody (titer, greater than or equal to 1:32) to H3N2 was significantly associated with protection from illness and infection with influenza A/H3N2.
103名幼儿通过鼻内接种，分别接种了甲型流感/加利福尼亚/10/78冷重组疫苗（每名儿童接种10(6.7) 50%组织培养感染剂量[TCID50]）、CR - 37（H1N1），或甲型流感/华盛顿/897/80冷重组疫苗（每名儿童接种10(6.5) TCID50）、CR - 48（H3N2）。疫苗耐受性良好。在接种CR - 37（H1N1）的51名儿童中，45名最初对此病毒血清学阴性；45名中的33名感染了疫苗病毒，这通过抗体滴度四倍升高或疫苗病毒排出得以表明。在接种CR - 48（H3N2）的52名儿童中，6名最初血清学阴性且全部被感染；46名最初血清学阳性，其中25名儿童抗体滴度出现四倍升高。在儿童接种疫苗后1至3个月，亨廷顿发生了甲型流感（以H3N2型为主）疫情。CR - 48（H3N2）疫苗组出现的发热性疾病明显少于CR - 37（H1N1）疫苗组。针对H3N2的血凝抑制抗体（滴度大于或等于1:32）与预防甲型流感H3N2疾病和感染显著相关。
