Willson J K, Bittner G, Borden E C
J Interferon Res. 1984 Fall;4(4):441-7. doi: 10.1089/jir.1984.4.441.
Fresh biopsies from 14 of 22 (64%) ovarian carcinomas cultivated in the human tumor stem cell assay (HTSCA) were sensitive (greater than 70% inhibition in cell growth) to human interferons (HuIFNs). To achieve 70% inhibition of colony growth, 500 units/ml of a naturally produced IFN-alpha or IFN-alpha A were required in 71% of the sensitive specimens. The antiproliferative potencies of five IFNs were evaluated including two native alpha IFNs, two highly purified cloned subtypes of IFN-alpha, IFN-alpha D and IFN-alpha A, and one native fibroblast-derived beta interferon (IFN-beta). The antiviral activity of the IFN-alpha as determined by a human cell target correlated with their relative antiproliferative action. IFN-alpha D had minimal inhibitory effect at the highest concentration tested, while three IFN-alpha with high antiviral activities were equivalent with respect to growth inhibition in the HTSCA. Although instability could not be eliminated as a contributing factor, IFN-beta had significantly less growth inhibitory potency for cells from ovarian cancers when compared simultaneously with native IFN-alpha in the human tumor stem cell assay (HTSCA). Assuming direct antiproliferative effects are primary, future clinical trials evaluating IFN-alpha in ovarian cancer may require high titers of IFN.
在人类肿瘤干细胞分析（HTSCA）中培养的22例卵巢癌中有14例（64%）的新鲜活检组织对人干扰素（HuIFNs）敏感（细胞生长抑制率大于70%）。在71%的敏感标本中，需要500单位/毫升天然产生的IFN-α或IFN-α A才能实现70%的集落生长抑制。评估了五种干扰素的抗增殖能力，包括两种天然α干扰素、两种高度纯化的克隆亚型IFN-α D和IFN-α A，以及一种天然成纤维细胞衍生的β干扰素（IFN-β）。通过人类细胞靶点测定的IFN-α的抗病毒活性与其相对抗增殖作用相关。在测试的最高浓度下，IFN-α D的抑制作用最小，而三种具有高抗病毒活性的IFN-α在HTSCA中的生长抑制作用相当。尽管不能排除不稳定性作为一个影响因素，但在人类肿瘤干细胞分析（HTSCA）中，与天然IFN-α同时比较时，IFN-β对卵巢癌细胞的生长抑制效力明显较低。假设直接抗增殖作用是主要的，未来评估IFN-α治疗卵巢癌的临床试验可能需要高滴度的IFN。
