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六种海洋鱼类的奇网和鳔腺上皮中的过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶

Catalase, glutathione peroxidase, and superoxide dismutase in the rete mirabile and gas gland epithelium of six species of marine fishes.

作者信息

Morris S M, Albright J T

出版信息

J Exp Zool. 1984 Oct;232(1):29-39. doi: 10.1002/jez.1402320105.

DOI:10.1002/jez.1402320105
PMID:6502092
Abstract

Rete mirabile and gas gland epithelium from the swim bladders of six species of marine fishes were assayed for catalase, glutathione peroxidase, and superoxide dismutase activity. Correlation of the results of these assays with measurements of the concentration of oxygen in the lumen of the normal steady state swim bladders revealed that swim bladders in species containing higher levels of oxygen also exhibited higher levels of superoxide dismutase activity in the rete mirabile/gas gland epithelium region. There appeared to be no correlation between oxygen concentration and the level of catalase or glutathione peroxidase activity. Induction of the inflatory reflex in Opsanus tau by a single deflation of the swim bladder resulted in an increase in the percent of oxygen in the swim bladder lumen 18 to 24 hours later, but this was not accompanied by any significant increases in antioxidant enzyme activity. Swim bladders that were deflated three times at 24-hour intervals showed further increases in oxygen concentration at the end of the 72-hour period but no alteration in superoxide dismutase activity.

摘要

对六种海鱼鳔的奇网和气体腺上皮进行了过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶活性测定。这些测定结果与正常稳态鳔腔内氧气浓度测量值的相关性表明,在奇网/气体腺上皮区域,氧气含量较高的物种的鳔也表现出较高水平的超氧化物歧化酶活性。氧气浓度与过氧化氢酶或谷胱甘肽过氧化物酶活性水平之间似乎没有相关性。通过单次排空奥氏躄鱼的鳔诱导充气反射,在18至24小时后鳔腔内氧气百分比增加,但这并未伴随抗氧化酶活性的任何显著增加。以24小时间隔排空三次的鳔在72小时结束时氧气浓度进一步增加,但超氧化物歧化酶活性没有改变。

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