采用高效液相色谱-紫外检测法测定毛细血管和静脉血浆中的布洛芬。

Determination of ibuprofen in capillary and venous plasma by high-performance liquid chromatography with ultraviolet detection.

作者信息

Albert K S, Raabe A, Garry M, Antal E J, Gillespie W R

出版信息

J Pharm Sci. 1984 Oct;73(10):1487-9. doi: 10.1002/jps.2600731043.

Abstract

A high-performance liquid chromatographic (HPLC) method is described which determines ibuprofen in human capillary or venous plasma. Ibuprofen plus the internal standard, flurbiprofen, were extracted from acidified plasma with pentane-ether, back-extracted into base, and then extracted into the pentane-ether solution after acidification of the aqueous phase. A reverse-phase octadecylsilane column with acetonitrile-water-phosphoric acid as mobile phase and UV detection provided a quantifiable peak for 1 microgram/mL of ibuprofen in 0.1 mL of plasma. Capillary and venous plasma level curves were virtually superimposable after administration of 400 mg of ibuprofen to four normal volunteers. No ibuprofen was detected in the saliva of the subjects.

摘要

描述了一种高效液相色谱（HPLC）方法，用于测定人毛细血管或静脉血浆中的布洛芬。布洛芬加内标氟比洛芬，用戊烷 - 乙醚从酸化血浆中萃取，反萃取到碱液中，然后在水相酸化后再萃取到戊烷 - 乙醚溶液中。以乙腈 - 水 - 磷酸为流动相的反相十八烷基硅烷柱和紫外检测，能在0.1 mL血浆中为1微克/毫升的布洛芬提供可定量的峰。给4名正常志愿者服用400毫克布洛芬后，毛细血管和静脉血浆水平曲线几乎完全重叠。在受试者的唾液中未检测到布洛芬。