Katoh-Semba R, Skaper S D, Varon S
J Neurosci Res. 1984;12(2-3):299-310. doi: 10.1002/jnr.490120217.
The effects of ganglioside GM1 on proliferation and neuritic growth of PC12 pheochromocytoma cells were studied in the presence and absence of nerve growth factor (NGF). In the absence of NGF, but not in its presence, a decrease in the total number of PC12 cells was first observed after 4-6 days of culture with 10(-6) M GM1 in 0.1% fetal calf serum, and with 10(-3) M GM1 on 10% serum. NGF, with or without GM1, limits cell growth to the first 4-6 days. GM1 enhanced neuritic recruitment with serum concentrations of 0.3% or more. Optimal neurite response varied from 10(-6) M GM1 with 0.3% serum to 10(-4) M GM1 with 10% serum. The influence of GM1 on neurites became more pronounced with increasing serum concentrations, becoming maximal with 1% or greater serum. Serum exhibited a concentration-dependent inhibitory influence (lag) on NGF-induced neuritic recruitment, which was abolished by GM1. Rates of neuritic recruitment following the lag were unaffected by GM1, while showing an inverse correlation with serum concentrations of 0.1-0.5%. Serum may delay the NGF-induced neuritic recruitment of PC12 cells by two independent mechanisms. These results suggest that GM1, in some manner, prevents the serum-induced delay in the onset of neuritic recruitment, rather than stimulating the rate at which it precedes.
在有和没有神经生长因子（NGF）的情况下，研究了神经节苷脂GM1对PC12嗜铬细胞瘤细胞增殖和神经突生长的影响。在没有NGF的情况下（但有NGF时则不然），在含有0.1%胎牛血清的培养基中用10⁻⁶ M GM1培养4 - 6天后，以及在含有10%血清的培养基中用10⁻³ M GM1培养时，首次观察到PC12细胞总数减少。无论有无GM1，NGF都将细胞生长限制在前4 - 6天。GM1在血清浓度为0.3%或更高时增强神经突募集。最佳神经突反应范围从含0.3%血清的10⁻⁶ M GM1到含10%血清的10⁻⁴ M GM1。随着血清浓度增加，GM1对神经突的影响变得更加明显，在血清浓度为1%或更高时达到最大。血清对NGF诱导的神经突募集表现出浓度依赖性抑制作用（延迟），而GM1可消除这种作用。延迟后的神经突募集速率不受GM1影响，但与0.1 - 0.5%的血清浓度呈负相关。血清可能通过两种独立机制延迟NGF诱导的PC12细胞神经突募集。这些结果表明，GM1以某种方式阻止了血清诱导的神经突募集起始延迟，而不是刺激其起始速率。
