Wright B E
J Theor Biol. 1984 Oct 5;110(3):445-60. doi: 10.1016/s0022-5193(84)80186-1.
The constraints in the parameters in models of the spore and stalk cells in Dictyostelium discoideum have been examined. It was found that the relative sizes of the two cellular glucose pools are not very critical, i.e. they can be varied in the models over a fairly wide range and still allow simulations which are compatible with the data. In contrast, the following model parameters are highly constrained, and must fall within narrow limits: flux through the glycogen cycle; the fraction of glycogen present which actually participates in glycogen turnover; the net rate of glycogen degradation; the concentration of exogenous labelled glucose which actually participates in cellular metabolism; the rates of exchange of this exogenous glucose with the two cellular glucose pools; the concentration of the spore glucose-6-phosphate pool, and the rate of exchange of stalk glucose-1-phosphate and stalk glucose-6-phosphate.
对盘基网柄菌中孢子细胞和柄细胞模型的参数约束进行了研究。结果发现，两个细胞葡萄糖池的相对大小并非非常关键，也就是说，在模型中它们可以在相当宽的范围内变化，并且仍然能够进行与数据相符的模拟。相比之下，以下模型参数受到高度约束，必须落在狭窄的范围内：通过糖原循环的通量；实际参与糖原周转的糖原所占比例；糖原降解的净速率；实际参与细胞代谢的外源性标记葡萄糖的浓度；这种外源性葡萄糖与两个细胞葡萄糖池的交换速率；孢子葡萄糖 -6-磷酸池的浓度，以及柄细胞葡萄糖 -1-磷酸和柄细胞葡萄糖 -6-磷酸的交换速率。
