Wright B E, Killick K A, Tai A
Eur J Biochem. 1977 Apr 1;74(2):217-25. doi: 10.1111/j.1432-1033.1977.tb11384.x.
The kinetic model of carbohydrate metabolism has been expanded to include: (a) the accumulation of alpha and beta-cellulose, insoluble cell-wall glycogen and mucopolysaccharide; (b) the role of RNA turnover as a source of carbon for end-product synthesis and as a buffer regulating the level of uridine nucleotides in this metabolic network; and (c) the role of purine-nucleoside phosphorylase, 5'-AMP nucleotidase, nucleosidediphosphate kinase and polynucleotide phosphorylase. One of many predictions based on this model is that cells differentiating in the presence of glucose will produce sorocarps with an abnormally high trehalose to cellulose ratio. External perturbation of either the model or of developing cells by glucose increases the levels of sorocarp trehalose and glycogen, 5-fold and 6-fold respectively. Evaluation of the experimental data and the simulation analyses have allowed several predictions to be made concerning the compartmentation of metabolites and the permeability of cells to glucose during differentiation.
碳水化合物代谢的动力学模型已得到扩展，包括：（a）α-纤维素和β-纤维素、不溶性细胞壁糖原和粘多糖的积累；（b）RNA周转作为终产物合成碳源以及作为调节该代谢网络中尿苷核苷酸水平的缓冲剂的作用；以及（c）嘌呤核苷磷酸化酶、5'-AMP核苷酸酶、核苷二磷酸激酶和多核苷酸磷酸化酶的作用。基于该模型的众多预测之一是，在葡萄糖存在下分化的细胞将产生海藻糖与纤维素比率异常高的子实体。通过葡萄糖对模型或发育中的细胞进行外部扰动，分别使子实体海藻糖和糖原水平增加5倍和6倍。对实验数据的评估和模拟分析使得能够对分化过程中代谢物的区室化以及细胞对葡萄糖的通透性做出若干预测。
