Sheffield L G, Anderson R R
Life Sci. 1984 Nov 26;35(22):2199-203. doi: 10.1016/0024-3205(84)90460-0.
In order to determine the effect of relaxin and estradiol on collagen and noncollagen synthesis by mammary gland fibroblasts, fibroblasts isolated from guinea pig mammary glands were grown on plastic or Cytodex-3 collagen coated microcarriers. On plastic, estradiol (600 pg/ml) increased the incorporation of tritiated glycine into collagen. Non-collagenous protein synthesis was increased at all concentrations of estradiol, but it was greatest with 200 pg/ml estradiol. On Cytodex-3, 400 pg/ml estradiol increased the synthesis of collagen and non-collagenous protein. Relaxin (1 microgram/ml) did not affect collagen synthesis but decreased the synthesis of non-collagenous protein.
为了确定松弛素和雌二醇对乳腺成纤维细胞胶原蛋白和非胶原蛋白合成的影响，从豚鼠乳腺分离出的成纤维细胞在塑料或涂有Cytodex-3胶原蛋白的微载体上培养。在塑料培养条件下，雌二醇（600 pg/ml）增加了氚标记甘氨酸掺入胶原蛋白的量。在所有雌二醇浓度下，非胶原蛋白合成均增加，但在200 pg/ml雌二醇时增加最多。在Cytodex-3上，400 pg/ml雌二醇增加了胶原蛋白和非胶原蛋白的合成。松弛素（1微克/毫升）不影响胶原蛋白合成，但降低了非胶原蛋白的合成。
