Huang C, Li Y, Anderson L L
Department of Animal Science, Iowa State University, Ames 50011-3150.
J Reprod Fertil. 1993 May;98(1):153-8. doi: 10.1530/jrf.0.0980153.
The role of relaxin and oestrogen in the remodelling of connective tissue was investigated by testing collagenase-dispersed cells (3 x 10(5) cells per well) from the uterine cervix of gilts for relaxin binding and collagen secretion. Relaxin-binding sites on these cells were quantified by specific binding of a saturating dose of 125I-labelled monotyrosyl relaxin at optimal conditions. Oestrogen at doses from 0.4 to 50 ng ml-1 increased relaxin binding in a time- and dose-dependent manner. Scatchard plot analysis exhibited curvilinearity, which suggested two classes of relaxin-binding sites. The addition of relaxin (0, 100, 500 ng ml-1) alone (P < 0.05) or in combination with oestrogen (oestradiol benzoate: 0, 50, 250 ng ml-1) increased protein secretion into the culture medium. Hydroxyproline concentration (as an index of collagen) in the medium was increased (P < 0.05) only in the presence of both relaxin and oestrogen. Actinomycin D (500 ng ml-1) and cycloheximide (500 ng ml-1) inhibited hydroxyproline secretion induced by combined relaxin and oestrogen treatment. Dibutyryl cyclic adenosine 3',5'-monophosphate (dibutyryl cAMP: 0, 0.1, 1.0, 5.0 mmol l-1) was a potent stimulator of hydroxyproline secretion. These results indicate that relaxin, probably via a cAMP pathway, stimulates hydroxyproline secretion in the presence of oestrogen through a protein- and RNA-synthesis dependent process. Oestrogen plays a role in augmenting the sensitivity of uterine cervical cells to relaxin in the pig.
通过检测来自后备母猪子宫颈的胶原酶分散细胞（每孔3×10⁵个细胞）的松弛素结合情况和胶原蛋白分泌，研究了松弛素和雌激素在结缔组织重塑中的作用。在最佳条件下，通过饱和剂量的¹²⁵I标记单酪氨酸松弛素的特异性结合来定量这些细胞上的松弛素结合位点。剂量为0.4至50 ng/ml的雌激素以时间和剂量依赖性方式增加松弛素结合。Scatchard图分析显示为曲线，这表明存在两类松弛素结合位点。单独添加松弛素（0、100、500 ng/ml）（P<0.05）或与雌激素（苯甲酸雌二醇：0、50、250 ng/ml）联合添加均可增加培养基中的蛋白质分泌。仅在同时存在松弛素和雌激素的情况下，培养基中羟脯氨酸浓度（作为胶原蛋白的指标）才会升高（P<0.05）。放线菌素D（500 ng/ml）和环己酰亚胺（500 ng/ml）可抑制松弛素和雌激素联合处理诱导的羟脯氨酸分泌。二丁酰环磷腺苷（二丁酰cAMP：0、0.1、1.0、5.0 mmol/l）是羟脯氨酸分泌的有效刺激剂。这些结果表明，松弛素可能通过cAMP途径，在雌激素存在的情况下，通过蛋白质和RNA合成依赖的过程刺激羟脯氨酸分泌。雌激素在增强猪子宫颈细胞对松弛素的敏感性方面发挥作用。
