Gerike L
Ukr Biokhim Zh. 1976;48(6):755-8.
Methods of double diffusion failed to detect differences in immunological properties of aldolase from muscles of normal rabbits and those fasting for a long time. Therefore a quantitative method was used to inhibit the aldolase activity by homologous and heterologous antisera. The data obtained evidence for appearance of additional antigen determinants in an algolase molecule of the animals fasting for a long time; the determinants being sterically connected with the site of the enzyme active centre.
双向扩散法未能检测出正常家兔肌肉和长期禁食家兔肌肉中醛缩酶免疫特性的差异。因此,采用定量方法用同源和异源抗血清抑制醛缩酶活性。所获数据证明,长期禁食动物的醛缩酶分子中出现了额外的抗原决定簇;这些决定簇在空间上与酶活性中心位点相连。