Stelzer K J, Gordon M A
Res Commun Chem Pathol Pharmacol. 1984 Oct;46(1):137-50.
The in vitro effects of pyrethroids on the mitogenic responsiveness of murine splenic lymphocytes to concanavalin A (Con A) and lipopolysaccharide (LPS) have been determined. Mitogenic stimulation was measured by uptake of [6-3H]-thymidine. Permethrin and cypermethrin inhibited the mitogenic response to Con A over a concentration range of 1 X 10(-5) M to 5 X 10(-5) M. Relative to these pyrethroids, slightly lower concentrations of fenpropathrin were required to inhibit mitogenic responsiveness to Con A. Fluvalinate was least effective of the pyrethroids tested, and caused mitogenic inhibition at concentrations of 1.5 X 10(-5) M to 5 X 10(-5) M. Unlike the other pyrethroids, concentrations of fluvalinate as high as 1 X 10(-4) M did not result in complete inhibition of the mitogenic response to Con A. Allethrin, was the most potent inhibitor, with effective concentrations in the range of 1 X 10(-6) M to 1.5 X 10(-5) M. Permethrin (3 X 10(-5) M) inhibited the mitogenic response at each of several concentrations of Con A tested, and did not cause a shift in the lymphocyte mitogenic dose-response to Con A. The mitogenic responsiveness to LPS was found to be inhibited by allethrin and permethrin at the same concentrations which inhibited the mitogenesis induced by Con A. The results support the possibility of immune suppression by pyrethroid exposure.
已测定拟除虫菊酯对小鼠脾淋巴细胞对刀豆蛋白A（Con A）和脂多糖（LPS）的促有丝分裂反应性的体外作用。通过[6-³H]胸腺嘧啶核苷的摄取来测量促有丝分裂刺激。氯菊酯和氯氰菊酯在1×10⁻⁵ M至5×10⁻⁵ M的浓度范围内抑制对Con A的促有丝分裂反应。相对于这些拟除虫菊酯，抑制对Con A的促有丝分裂反应性所需的甲氰菊酯浓度略低。氟胺氰菊酯在所测试的拟除虫菊酯中效果最差，在1.5×10⁻⁵ M至5×10⁻⁵ M的浓度下引起促有丝分裂抑制。与其他拟除虫菊酯不同，高达1×10⁻⁴ M的氟胺氰菊酯浓度并未导致对Con A的促有丝分裂反应完全抑制。丙烯菊酯是最有效的抑制剂，有效浓度范围为1×10⁻⁶ M至1.5×10⁻⁵ M。氯菊酯（3×10⁻⁵ M）在测试的几种Con A浓度下均抑制促有丝分裂反应，并且未导致淋巴细胞促有丝分裂剂量反应对Con A的偏移。发现对LPS的促有丝分裂反应性在抑制由Con A诱导的有丝分裂的相同浓度下被丙烯菊酯和氯菊酯抑制。结果支持拟除虫菊酯暴露导致免疫抑制的可能性。
