一种利用丹磺酰尸胺掺入和凝胶过滤的新型快速简便的因子 XIII 活性检测方法。

A new rapid and simple assay for factor XIII activity using dansylcadaverine incorporation and gel filtration.

作者信息

Nishida Y, Ikematsu S, Fukutake K, Fujimaki M, Fukutake K, Kakishita E

出版信息

Thromb Res. 1984 Oct 15;36(2):123-31. doi: 10.1016/0049-3848(84)90334-7.

Abstract

A new sensitive, reproducible and simple assay for factor XIII activity was developed, in which fluorescent dansylcadaverine (DC) is incorporated into casein and DC-casein is separated from free DC by gel filtration. A good correlation was found between our method and Lorand's. The main advantages of our method are: (1) our assay technique is simple with good reproducibility and takes only a couple of hours; (2) the detectable limit of factor XIII activity is about 1% of normal pool plasma, but, if necessary, can be lowered by increasing the sample amount and lengthening the reaction time.

摘要

开发了一种新的用于检测因子 XIII 活性的灵敏、可重复且简单的检测方法，该方法中，荧光丹磺酰尸胺（DC）被掺入酪蛋白中，并且通过凝胶过滤将 DC-酪蛋白与游离 DC 分离。我们的方法与罗兰德的方法之间发现有良好的相关性。我们方法的主要优点是：（1）我们的检测技术简单，具有良好的可重复性，只需几个小时；（2）因子 XIII 活性的可检测限约为正常混合血浆的 1%，但如有必要，可通过增加样品量和延长反应时间来降低检测限。