Lau S S, Monks T J, Greene K E, Gillette J R
Xenobiotica. 1984 Jul;14(7):539-43. doi: 10.3109/00498258409151444.
Bromobenzene-3,4-oxide can be detected in venous blood of rats by trapping it as the corresponding 35[S]glutathione conjugates. More bromobenzene-3,4-oxide is detected in venous blood of rats treated with phenobarbital and diethyl maleate than in venous blood of rats treated with phenobarbital alone. The half-life of bromobenzene-3,4-oxide in venous blood was about 13.5 s. Bromobenzene-3,4-oxide may contribute to the extrahepatic covalent binding and presumably the toxicity observed after bromobenzene administration. The present technique may be used to determine in blood, the presence or absence of other reactive metabolites that form glutathione conjugates.
通过将溴苯 -3,4- 氧化物捕获为相应的 35[S] 谷胱甘肽共轭物，可以在大鼠的静脉血中检测到它。用苯巴比妥和马来酸二乙酯处理的大鼠静脉血中检测到的溴苯 -3,4- 氧化物比仅用苯巴比妥处理的大鼠静脉血中更多。溴苯 -3,4- 氧化物在静脉血中的半衰期约为 13.5 秒。溴苯 -3,4- 氧化物可能导致肝外共价结合，并可能导致溴苯给药后观察到的毒性。本技术可用于确定血液中是否存在其他形成谷胱甘肽共轭物的活性代谢物。
