Fox C H, Auer G, Zetterberg A, Cottler-Fox M
J Microsc. 1978 Mar;112(2):239-42. doi: 10.1111/j.1365-2818.1978.tb01171.x.
An inexpensive culture chamber suitable for subsequent preparations for cytochemical or radiographic work is demonstrated. The chamber consists of two glass microscope slides suitably prepared for cell growth and a gasket of silicone rubber. The slides and gasket are sealed and held together with two ordinary spring letter clips or clothes-pins. Cells may be inoculated by piercing the gasket with a hypodermic needle, or by removing the top slide and adding cells directly. Long-term cultivation with medium replacement is easy and efficient and addition of isotopes or other materials may be done aseptically.
展示了一种适用于后续细胞化学或放射学工作准备的廉价培养室。该培养室由两片经适当处理以促进细胞生长的玻璃显微镜载玻片和一个硅橡胶垫圈组成。载玻片和垫圈用两个普通的弹簧夹或衣夹密封并固定在一起。细胞可以通过用皮下注射针刺穿垫圈来接种，也可以通过取下上面的载玻片并直接添加细胞来接种。通过更换培养基进行长期培养简便高效，并且可以无菌添加同位素或其他物质。
