Boocock C A, Brown A F, Dunn G A
J Microsc. 1985 Jan;137(Pt 1):29-34. doi: 10.1111/j.1365-2818.1985.tb02558.x.
A method is described which permits the observation of microscopic specimens in both top and side views, a rapid change between the two views being possible without disturbing the specimen. The system requires the introduction of simple optical components into a culture chamber, does not restrict the types of microscopy that may be used, and does not necessitate modification of the microscope itself. The technique is discussed in relation to the study of tissue cells in culture, but may find applications in other areas.
本文描述了一种方法,该方法允许从顶部和侧面观察微观标本,并且能够在不干扰标本的情况下快速在两种视图之间切换。该系统只需在培养室中引入简单的光学组件,不限制可使用的显微镜类型,也无需对显微镜本身进行改装。本文讨论了该技术在培养组织细胞研究中的应用,但它可能在其他领域也有应用。