Onwuliri C O
Z Parasitenkd. 1984;70(5):667-71. doi: 10.1007/BF00926596.
The adenosine triphosphate (ATP) content of the pre-parasitic stages of Nippostrongylus brasiliensis, Haemonchus contortus (L1, L2 and L3) and the adults of the free-living nematode, Panagrellus redivivus, have been measured by bioluminescent photometry in aerated or near-anoxic conditions. The ATP content of the L1 and L2 stages of both parasitic species was unaltered by a lack of oxygen over a 90-min period. However, the L3 stage of both species and the adults of P. redivivus showed a significant fall in the level of ATP within 10 min of near-anoxia. This lower level of ATP was maintained during oxygen lack but the initial content was restored on return of the nematodes to aerobic conditions. The results suggest that measurement of ATP by bioluminescent photometry offers a readily measured and sensitive indicator of the capacity of a nematode to cope with transient changes in oxygen supply without undue metabolic stress.
利用生物发光光度法，在通气或近缺氧条件下，测定了巴西日圆线虫、捻转血矛线虫（L1、L2和L3期）的寄生前期以及自由生活线虫复苏短体线虫成虫的三磷酸腺苷（ATP）含量。在90分钟内，缺氧对两种寄生线虫L1和L2期的ATP含量没有影响。然而，两种线虫的L3期以及复苏短体线虫成虫在近缺氧10分钟内，ATP水平显著下降。在缺氧期间，ATP水平维持在较低水平，但线虫恢复到有氧条件后，初始含量得以恢复。结果表明，通过生物发光光度法测量ATP，为线虫应对氧气供应瞬态变化而不产生过度代谢应激的能力，提供了一种易于测量且灵敏的指标。
