Aguilar-Marcelino Liliana, Mendoza-de-Gives Pedro, Torres-Hernández Glafiro, López-Arellano María Eugenia, González-Garduño Roberto
Centro Nacional de Investigación Disciplinaria en Salud Animal E Inocuidad, (CENID-SAI) INIFAP, Unidad de Helmintología, Boulevard Paseo Cuauhnahuac No. 8534, Col. Progreso, Jiutepec, Morelos, CP 62550, México.
Colegio de Postgraduados, Estado de México, México.
Acta Parasitol. 2020 Dec;65(4):865-873. doi: 10.2478/s11686-020-00216-z. Epub 2020 Jun 5.
This study assessed the feeding activity of the predatory nematode Butlerius butleri on the infective larvae of the sheep parasitic nematode Haemonchus contortus (Hc-L) and on two free-living nematodes Panagrellus redivivus (Pr) and Rhabditis sp. (R) in sterile sheep faecal cultures.
A bioassay was carried out in sheep faecal cultures, where predatory and prey nematodes were put together to assess the predatory behaviour on the different nematodes. Faecal cultures (n = 9) were set up in different treatments as follows: Treatments 1, 2 and 3 contained 20 specimens of B. butleri and 2000 Hc-L, Pr and R, respectively; treatments 4, 5 and 6, contained only 2000 Hc-L, Pr and R, respectively, as control groups. Treatment 7 contained 20 B. butleri as control. All treatments were incubated at room temperature for 30 days. The average number of nematodes recovered from each treatment was obtained and the reduction rate with respect to their corresponding control was estimated. Data were analysed using an ANOVA test using a completely randomised design in the SAS programme.
Butlerius butleri reduced the Hc-L, population by 91.8%, whereas its population increased 131.6 times. The interactions between B. butleri and Pr and R resulted in 80.9% and 62.5% nematode reduction and B. butleri increased its populations 268.3 and 82.7 times, respectively. This experiment was performed only once, so this can be considered a preliminary report at this time.
This study provides evidence for the first time that B. butleri feeds on one of the most economically important nematodes of small ruminants, Hc-L.
本研究评估了捕食性线虫巴氏布特勒线虫对绵羊寄生线虫捻转血矛线虫感染性幼虫（Hc-L）以及两种自由生活线虫重生滑刃线虫（Pr）和小杆线虫属（R）在无菌绵羊粪便培养物中的捕食活性。
在绵羊粪便培养物中进行了一项生物测定，将捕食性线虫和猎物线虫放在一起，以评估对不同线虫的捕食行为。粪便培养物（n = 9）设置了不同处理如下：处理1、2和3分别包含20条巴氏布特勒线虫和2000条Hc-L、Pr和R；处理4、5和6分别仅包含2000条Hc-L、Pr和R作为对照组。处理7包含20条巴氏布特勒线虫作为对照。所有处理在室温下孵育30天。获得从每个处理中回收的线虫平均数量，并估计相对于其相应对照的减少率。使用SAS程序中的完全随机设计通过方差分析检验对数据进行分析。
巴氏布特勒线虫使Hc-L种群减少了91.8%，而其种群数量增加了131.6倍。巴氏布特勒线虫与Pr和R之间的相互作用导致线虫减少了80.9%和62.5%，并且巴氏布特勒线虫的种群数量分别增加了268.3倍和82.7倍。该实验仅进行了一次，所以此时可将其视为初步报告。
本研究首次提供证据表明巴氏布特勒线虫以小型反刍动物最具经济重要性的线虫之一Hc-L为食。
