Liebert C A, Hood M A, Deck F H, Bishop K, Flaherty D K
Appl Environ Microbiol. 1984 Nov;48(5):936-43. doi: 10.1128/aem.48.5.936-943.1984.
A yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with hypersensitivity pneumonitis-like symptoms. The bacterium, designated WF-164, lacked microcysts or fruiting bodies and had a DNA base composition of 34.8 mol% of guanine plus cytosine. Gliding, flexing, nonflagellated cells measuring 0.3 by 3.5 to 8.9 micron were observed by using light and electron microscopy. Tests to determine utilization of selected carbohydrates revealed an amylolitic, chitinoclastic, noncellulytic bacterium. A number of additional biochemical and physiological tests were performed. DNA homology studies detected a 77.8% similarity to Cytophaga aquatilis (ATCC 29551). Comparisons of cellular fatty acid and carbohydrate contents of isolate WF-164 with a Flexibacter sp., several Cytophaga spp., and Flavobacterium reference strains revealed similar patterns to that of C. aquatilis. On the basis of these characteristics, isolate WF-164 was identified as a new Cytophaga sp.
从一个工业喷水空气加湿系统中分离出的一种黄色色素沉着、革兰氏阴性、滑行细菌被认为是几起伴有类过敏性肺炎症状的肺部疾病的病原体。这种细菌被命名为WF - 164，没有微囊肿或子实体，鸟嘌呤加胞嘧啶的DNA碱基组成为34.8摩尔%。通过光学显微镜和电子显微镜观察到，其滑行、弯曲、无鞭毛的细胞大小为0.3×3.5至8.9微米。用于确定所选碳水化合物利用情况的测试表明这是一种分解淀粉、分解几丁质、不分解纤维素的细菌。还进行了许多其他的生化和生理测试。DNA同源性研究检测到它与水生噬纤维菌（ATCC 29551）有77.8%的相似性。将分离株WF - 164的细胞脂肪酸和碳水化合物含量与一种屈挠杆菌属细菌、几种噬纤维菌属细菌以及黄杆菌属参考菌株进行比较，发现其模式与水生噬纤维菌相似。基于这些特征，分离株WF - 164被鉴定为一种新的噬纤维菌属细菌。
