Donato R
Biochem Biophys Res Commun. 1984 Nov 14;124(3):850-6. doi: 10.1016/0006-291x(84)91035-0.
The inhibitory effect of S-100 on microtubule protein assembly is inversely related to the microtubule protein concentration and/or the temperature of assembly. Moreover, the S-100-induced decrease in the rate and extent of assembly is positively correlated with the length of the lag of assembly. When microtubule fragments are added to the microtubule protein solution, the S-100 effect is reduced but not abolished. These data suggest that S-100 interferes with both the nucleation and the elongation of microtubules. Since S-100 also inhibits the assembly of purified tubulin, S-100 is suggested to affect the microtubule assembly by interacting with and sequestering tubulin.
S - 100对微管蛋白组装的抑制作用与微管蛋白浓度和/或组装温度呈负相关。此外，S - 100诱导的组装速率和程度降低与组装延迟的长度呈正相关。当将微管片段添加到微管蛋白溶液中时，S - 100的作用会降低但不会消除。这些数据表明S - 100会干扰微管的成核和延长过程。由于S - 100也抑制纯化微管蛋白的组装，因此提示S - 100通过与微管蛋白相互作用并隔离微管蛋白来影响微管组装。
