Dufourc E J, Smith I C, Jarrell H C
Biochim Biophys Acta. 1984 Dec 19;778(3):435-42. doi: 10.1016/0005-2736(84)90391-2.
The effects of amphotericin B upon the organization and dynamics of multibilayer membranes of dimyristoylphosphatidylcholine (DMPC) were investigated by means of 2H-NMR. At high amphotericin B concentrations (30 mol% with respect to the lipid) and at temperatures above 25 degrees C, DMPC experiences two different environments which are in slow exchange on the 2H-NMR time scale. In one of these, the lipid is immobilized by the antibiotic, in a molar ratio of approximately 1:1, whereas the lipid unsequestered by amphotericin B is more ordered than in its pure state. This ordering effect is perceived at relatively low antibiotic doses (4%). The local lipid order, and the relative percentage, of sequestered DMPC, are temperature-independent (up to 65 degrees C), whereas the ordering of the unsequestered lipid domain is not. The perturbation induced by amphotericin B is manifest similarly at the edges as well as in the center of the bilayer. Antibiotic addition leads to large decreases in the transverse relaxation time, T2, of the labelled lipid, but not in the spin-lattice relaxation time, T1. This indicates an increased density of slow motional modes and little change in rapid motions.
通过2H-核磁共振研究了两性霉素B对二肉豆蔻酰磷脂酰胆碱（DMPC）多层膜的结构和动力学的影响。在高两性霉素B浓度（相对于脂质为30摩尔%）且温度高于25℃时，DMPC经历两种不同的环境，它们在2H-核磁共振时间尺度上进行缓慢交换。在其中一种环境中，脂质以大约1:1的摩尔比被抗生素固定，而未被两性霉素B隔离的脂质比其纯态时更加有序。在相对低的抗生素剂量（4%）下就能察觉到这种有序化效应。被隔离的DMPC的局部脂质有序度和相对百分比与温度无关（高达65℃），而未被隔离的脂质区域的有序化则与温度有关。两性霉素B引起的扰动在双层膜的边缘和中心表现相似。添加抗生素会导致标记脂质的横向弛豫时间T2大幅降低，但自旋晶格弛豫时间T1没有变化。这表明慢运动模式的密度增加，而快速运动变化不大。
