Volkova N P, Lankin V Z
Biull Eksp Biol Med. 1984 Nov;98(11):546-8.
A study was made of variation in the activity of the antioxidant defense system enzymes superoxide dismitase (SOD), glutathione peroxidase, (GP) and glutathione-S-transferase (GT) in the cells of mouse spermatogenic epithelium. The cells were fractionated in the gradient of human serum albumin using the STAPUT system. SOD activity was comparable to that in liver cells, that of GP was one order to magnitude lower, and that of GT one order of magnitude higher than in the liver. Differentiation of spermatogenic cells demonstrated phase variations in the activity of these enzymes, with a maximum seen at the stage of late pachytene spermatocytes. SOD, GP and GT activities were discovered to be reduced in postmeiotic cells (early spermatid fractions) by 2.8-3.5; 1.9-2.3 and 4.2-5.6 times, respectively. That reduction was followed by activation of the enzymes at the late stages of spermiogenesis.
对小鼠生精上皮细胞中抗氧化防御系统酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GP）和谷胱甘肽-S-转移酶（GT）的活性变化进行了研究。使用STAPUT系统在人血清白蛋白梯度中对细胞进行分级分离。SOD活性与肝细胞中的相当，GP活性比肝细胞中的低一个数量级，而GT活性比肝细胞中的高一个数量级。生精细胞的分化显示出这些酶活性的阶段性变化，在粗线期晚期精母细胞阶段达到最大值。发现减数分裂后细胞（早期精子细胞组分）中SOD、GP和GT活性分别降低了2.8 - 3.5倍、1.9 - 2.3倍和4.2 - 5.6倍。在精子发生后期，这些酶的活性随后被激活。
