Viktorov I V, Shashkova N A
Biull Eksp Biol Med. 1984 Dec;98(12):729-31.
Dopaminergic neurons of Substantia nigra of 18-19-day-old mouse embryos were dissociated and cultured in Maximov's chambers for 2 weeks. The neurons showed an active synthesis of dopamine beginning from the first week of cultivation as demonstrated by the histofluorescent method. It is suggested that the process of differentiation of dopaminergic neurons is relatively independent of target cells and is genetically determined. Nonuniform distribution of dopaminergic neurons in aggregates suggests that these neurons are capable of selective adhesion.
将18 - 19日龄小鼠胚胎黑质中的多巴胺能神经元解离，并在马克西莫夫培养室中培养2周。组织荧光法显示，从培养的第一周开始，这些神经元就表现出活跃的多巴胺合成。这表明多巴胺能神经元的分化过程相对独立于靶细胞，且由基因决定。多巴胺能神经元在聚集体中的分布不均表明这些神经元具有选择性黏附能力。
