Bagnara G P, Valvassori L, Marini M, Franceschi C, Serafini-Cessi F
Cell Differ. 1984 Oct;14(4):277-85. doi: 10.1016/0045-6039(84)90016-2.
Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.
将小鼠骨髓细胞在含有骨髓来源的成纤维细胞和商陆刺激的脾条件培养基的琼脂中培养。诱导产生粒-单核细胞集落以及一些巨核细胞集落和混合集落。添加Tamm-Horsfall糖蛋白（一种在肾脏中产生的人糖蛋白）可增加集落数量并刺激巨核细胞生成。该糖蛋白在单层培养中没有集落刺激活性，也不诱导巨核细胞生成。文中讨论了造血微环境中发生的细胞间相互作用以及糖类的作用。
