Nusgens B, Merrill C, Lapiere C, Bell E
Coll Relat Res. 1984 Oct;4(5):351-63. doi: 10.1016/s0174-173x(84)80003-5.
Collagen biosynthesis by fibroblasts and subsequent processing and polymerization have been studied in conventional monolayer cultures and in a new model system in vitro in which cells organize into a tissue like structure. While the bulk of the newly made collagen becomes tightly and selectively bound to the matrix of the tissue equivalent model, in monolayer cultures most of the collagen passes into the culture medium. Collagen biosynthesis appears to be regulated differently in cells of the tissue equivalent model as compared with monolayered cells. In the former there is a 6- to 8-fold decrease in collagen output even though overall protein synthesis per unit of DNA is twice as great as in monolayered cells. Cells grown in tissue lattices exhibit much higher collagenolytic activity than cells in monolayer suggesting the model may also be of special use for studying collagen turnover and matrix remodeling.
成纤维细胞的胶原蛋白生物合成以及随后的加工和聚合过程，已在传统的单层培养物和一种新的体外模型系统中进行了研究，在该模型系统中细胞组织形成类似组织的结构。虽然新合成的大部分胶原蛋白紧密且选择性地结合到组织等效模型的基质上，但在单层培养中，大部分胶原蛋白进入培养基。与单层细胞相比，胶原蛋白生物合成在组织等效模型的细胞中似乎受到不同的调节。在前者中，胶原蛋白产量下降了6至8倍，尽管每单位DNA的总蛋白质合成是单层细胞的两倍。在组织晶格中生长的细胞比单层细胞表现出更高的胶原酶活性，这表明该模型对于研究胶原蛋白周转和基质重塑可能也有特殊用途。
