从虹鳟鱼中分离出的甘油醛-3-磷酸脱氢酶（EC 1.2.1.12）在5或15摄氏度适应过程中的修饰——I. 单个肌肉分离物活性的变化作为可变适应性反应的证据
Modification of glyceraldehyde-3-phosphate dehydrogenase, EC 1.2.1.12, isolated from rainbow trout during acclimation at 5 or 15 degrees C--I. Changes in the activity of individual muscle isolates as evidence for variable adaptive responses.
作者信息
Phelan M, Gray I
出版信息
Comp Biochem Physiol B. 1984;79(2):135-8. doi: 10.1016/0305-0491(84)90003-8.
The mean Km and Vmax values for G3PDH isolated from the lateral muscle of cold-adapted (5 degrees C) rainbow trout, Salmo gairdneri, were twice those of enzyme from warm-adapted (15 degrees C) trout when assayed at 7 degrees C but not at any other temperature. The entropy of activation of warm enzyme was about 3 times that of cold enzyme. However, enthalpy or free energy of activation among acclimation groups differed less or not at all. Individual G3PDH isolates within either adaptation group differed in kinetic characteristics.
从适应低温（5摄氏度）的虹鳟鱼（Salmo gairdneri）侧肌中分离出的甘油醛-3-磷酸脱氢酶（G3PDH），在7摄氏度下测定时，其平均米氏常数（Km）和最大反应速度（Vmax）值是从适应温暖环境（15摄氏度）的虹鳟鱼中分离出的该酶的两倍，但在其他任何温度下测定时并非如此。温暖环境下该酶的活化熵约为低温环境下该酶的3倍。然而，不同适应组之间的活化焓或活化自由能差异较小或根本没有差异。任一适应组内的单个G3PDH分离物在动力学特征上存在差异。
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