Seibert H
Comp Biochem Physiol B. 1985;81(4):877-83. doi: 10.1016/0305-0491(85)90082-3.
Endogenous glucose release and glycogen metabolism were investigated in isolated hepatocytes from rainbow trout acclimated to 10 and 20 degrees C. Thermal acclimation did not significantly affect hepatocyte glycogen contents and the rates of glucose release during substrate-free incubations. In both acclimation groups glucose production and glycogen metabolism exhibited clearly different dependencies on assay temperature. It was concluded, that there are different sources of glucose release in the lower and upper temperature range--gluconeogenesis from endogenous precursors at low temperatures and glycogenolysis at high temperatures. This conclusion was supported by experiments with 3-mercaptopicolinic acid, which stimulated glycogen breakdown especially in the low temperature range.
研究了适应10摄氏度和20摄氏度的虹鳟鱼分离肝细胞中的内源性葡萄糖释放和糖原代谢。热适应对肝细胞糖原含量以及无底物孵育期间的葡萄糖释放速率没有显著影响。在两个适应组中，葡萄糖生成和糖原代谢对测定温度表现出明显不同的依赖性。得出的结论是，在较低和较高温度范围内存在不同的葡萄糖释放来源——低温下由内源性前体进行糖异生，高温下进行糖原分解。用3-巯基吡啶甲酸进行的实验支持了这一结论，该物质尤其在低温范围内刺激糖原分解。
