[Lewis肺癌细胞在扩散小室琼脂培养中的克隆]
[Cloning of Lewis lung carcinoma cells in agar cultures in diffusion chambers].
作者信息
Kolesnikova A I, Lepekhina L A, Konopliannikov A G
出版信息
Eksp Onkol. 1984;6(2):32-5.
PMID:6510332
Abstract
A method was developed for cloning the Lewis lung carcinoma cells (LLC) in vivo in diffusion chambers. This method is analogous to the system introduced by Gordon for cloning hemopoietic precursor cells. No significant differences were observed in the efficiency of the cloning of the tumour cells within the range of 1 to 4 weeks after transplantation (7.7 +/- 0.7% for 240 cultures).
摘要
开发了一种在扩散室中对Lewis肺癌细胞（LLC）进行体内克隆的方法。该方法类似于Gordon引入的用于克隆造血前体细胞的系统。移植后1至4周内，肿瘤细胞的克隆效率未观察到显著差异（240个培养物的克隆效率为7.7±0.7%）。
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