Ito F, Chou J Y
J Biol Chem. 1984 Dec 25;259(24):14997-9.
Placental alkaline phosphatase activity and immunoreactivity were inhibited in a parallel fashion in choriocarcinoma cells by tunicamycin, a protein glycosylation inhibitor. Tunicamycin suppressed placental alkaline phosphatase biosynthesis in addition to inhibiting protein glycosylation in general. An anti-placental alkaline phosphatase-precipitable polypeptide of 58,000 daltons was formed in the presence of this antibiotic. The 58,000-dalton polypeptide had a degradation rate similar to that of the glycosylated phosphatase monomer from control cultures. Tunicamycin suppressed placental alkaline phosphatase mRNA activity leading to the observed decrease in biosynthesis.
蛋白质糖基化抑制剂衣霉素可使绒毛膜癌细胞中的胎盘碱性磷酸酶活性和免疫反应性呈平行方式受到抑制。衣霉素除了一般地抑制蛋白质糖基化外，还抑制胎盘碱性磷酸酶的生物合成。在这种抗生素存在的情况下，形成了一种58,000道尔顿的抗胎盘碱性磷酸酶可沉淀多肽。这种58,000道尔顿的多肽的降解速率与对照培养物中糖基化磷酸酶单体的降解速率相似。衣霉素抑制胎盘碱性磷酸酶mRNA活性，导致观察到的生物合成减少。
