Davis P J, Nail B S
J Comp Neurol. 1984 Nov 20;230(1):13-32. doi: 10.1002/cne.902300103.
Motoneurons supplying the posterior crico-arytenoid (PCA), thyro-arytenoid (TA), lateral crico-arytenoid (LCA), and crico-thyroid (CT) laryngeal muscles were localized in the cat, the rabbit, and the 6-week-old kitten by using the technique of intramuscular injection of horseradish peroxidase. Each muscle was found to be innervated by a single, ipsilateral pool of motoneurons, a result which was reliably established only after controlling adventitious spread of the label to nontarget muscles by prior denervation of adjacent musculature. The laryngeal motoneuron column extended in the nucleus ambiguus for a distance of 5-6 mm caudally from the facial nucleus. CT motoneurons were located in the rostral third of this column while the PCA, TA, and LCA motoneurons were located more caudally. These results are in general agreement with earlier degeneration studies (Lawn, '66a; Szentágothai, '43). Although labelled cells were widely dispersed in the nucleus, particularly in the adult cat, a limited amount of topographical structure could still be discerned in the arrangement of recurrent laryngeal nerve motoneurons. In the cat, the PCA pool was located in the ventral part of the recurrent laryngeal nerve representation and did not extend as far caudally as the TA or LCA pools; the LCA pool was located in the caudal and dorsomedial part of the recurrent laryngeal nerve pool; TA motoneurons appeared to overlap the PCA and LCA pools on all three anatomical planes. TA motoneurons were more numerous than PCA or LCA motoneurons, the numbers of cells in the three pools being estimated at 170, 111, and 112, respectively. In the cat bilateral labelling of different pools pointed to certain differences in morphology between cells from these pools and also suggested a functional basis for such differences. The mean soma diameter for the PCA and CT motoneurons was each significantly smaller than that for the TA and LCA motoneurons. The rabbit data were similar. The findings on motoneuron morphology are considered in relation to anatomical and physiological characteristics known to have been established for individual laryngeal muscles and with which they appear to be consistent.
通过肌肉内注射辣根过氧化物酶技术，在猫、兔和6周龄小猫中定位了支配环杓后肌（PCA）、甲杓肌（TA）、环杓侧肌（LCA）和环甲肌（CT）的运动神经元。发现每块肌肉均由单个同侧运动神经元池支配，只有在通过预先去神经支配相邻肌肉组织来控制标记物向非靶肌肉的意外扩散之后，才能可靠地得出这一结果。喉运动神经元柱在疑核中从面神经核向后延伸5-6毫米。CT运动神经元位于该柱的前三分之一，而PCA、TA和LCA运动神经元位于更靠后的位置。这些结果与早期的变性研究（Lawn，'66a；Szentágothai，'43）总体一致。尽管标记细胞在核内广泛分散，尤其是在成年猫中，但在喉返神经运动神经元的排列中仍可辨别出有限的拓扑结构。在猫中，PCA运动神经元池位于喉返神经代表区的腹侧部分，不像TA或LCA运动神经元池那样向尾侧延伸那么远；LCA运动神经元池位于喉返神经池的尾侧和背内侧部分；TA运动神经元在所有三个解剖平面上似乎与PCA和LCA运动神经元池重叠。TA运动神经元比PCA或LCA运动神经元数量更多，三个运动神经元池中的细胞数量估计分别为170、111和112。在猫中，不同运动神经元池的双侧标记表明这些运动神经元池的细胞在形态上存在某些差异，也暗示了这种差异的功能基础。PCA和CT运动神经元的平均胞体直径均显著小于TA和LCA运动神经元。兔的数据与之相似。结合已知为各个喉肌所确立的解剖和生理特征，对运动神经元形态学的研究结果进行了考量，这些结果似乎与之相符。
