Sakai C, Yamamoto T, Kitawaki J, Yoshiji S, Yasuda J, Yamaki J, Fujii M, Tsukamoto K, Honjo H, Okada H
J Endocrinol Invest. 1984 Oct;7(5):441-4. doi: 10.1007/BF03348447.
An equimolar mixture of 3H-E1-S2 and 14C-E1 was injected in one shot into the inferior vena cava near the heart of female Japanese monkey. Following the injection, blood was collected from the aortic arch at intervals of 15 s over a period of 10 min. The concentration of radioactivities in the whole blood and serum was measured. The metabolites were analyzed by DEAE-Sephadex A-25 column chromatography, enzyme hydrolysis, thin layer chromatography and paper chromatography. Both radioactivities of 3H-E1-S and 14C-E1 rapidly decreased in the first 90-s serum sample. The 3H/14C ratio in the 0-15-s serum sample was 5 times higher than the initial ratio of injected compounds. The radioactivities in the serum gradually decreased after 90 s of injection. The 3H/14C ratio in the pulmonary tissue was very low after collecting the final blood sample. This result shows that the most of 3H-E1-S passed through the lung and the larger part of 14C-E1 remained in the lung following injection of these materials. So, it is probable that E1-S is in a form to be carried in the general circulation.
将3H-E1-S2和14C-E1的等摩尔混合物一次性注入雌性日本猕猴心脏附近的下腔静脉。注射后，在10分钟内每隔15秒从主动脉弓采集血液。测量全血和血清中的放射性浓度。通过DEAE-葡聚糖A-25柱色谱、酶水解、薄层色谱和纸色谱分析代谢物。在最初的90秒血清样本中，3H-E1-S和14C-E1的放射性均迅速下降。0-15秒血清样本中的3H/14C比值比注射化合物的初始比值高5倍。注射90秒后，血清中的放射性逐渐下降。采集最后一份血液样本后，肺组织中的3H/14C比值非常低。该结果表明，注射这些物质后，大部分3H-E1-S通过了肺部，而大部分14C-E1留在了肺部。因此，E1-S很可能是以一种可在体循环中携带的形式存在。
