Patel S T, Newman H A, Yates A J, Thibert P, Falko J M
J Lipid Res. 1984 Oct;25(10):1072-83.
Serum lipid and lipoprotein composition in spontaneously diabetic BB Wistar rats, nondiabetic littermates, and control Wistar rats was studied to elucidate diabetes-related abnormalities of lipoprotein composition. Serum total triglycerides and pre-beta-lipoprotein concentrations of insulin-treated spontaneously diabetic BB and nondiabetic littermate rats were significantly higher than those of control Wistar rats. Serum cholesterol and HDL cholesterol concentrations of spontaneously diabetic BB and nondiabetic littermate rats did not differ from controls. Concentrations of very low density lipoproteins (VLDL), low density lipoproteins (LDL), and high density lipoproteins (HDL) of spontaneously diabetic BB and nondiabetic littermate rats were higher than those of normal rats. With sodium dodecylsulfate-polyacrylamide gel electrophoresis it was observed that the spontaneously diabetic BB and nondiabetic littermate rat VLDL contained higher percentages of apoE relative to total apoC when compared with control Wistar rats. With isoelectric focusing, apoC-II relative percentages in VLDL and HDL of both spontaneously diabetic BB and nondiabetic littermate rats were higher than apoC-II proportions in VLDL and HDL of controls. Apolipoprotein A-I of the control rat HDL showed four isoforms that focused at pI 5.8 (17.3%), 5.75 (30.6%), 5.65 (31.8%), and 5.55 (20.5%); however, the spontaneously diabetic BB and nondiabetic littermate rat HDL apoA-I was mainly represented by two isoforms that focused at pI 5.8 and 5.75. VLDL of both diabetic and nondiabetic BB rats contained higher levels of acidic apoE isoforms compared to their counterparts in control Wistar rats. Although HDL cholesterol concentrations of spontaneously diabetic BB rats remained normal, protein concentrations were higher resulting in a low cholesterol/protein ratio in HDL suggesting that the cholesterol-carrying capacity of spontaneously diabetic BB rat HDL could be less than normal and may be due to an abnormal apoA-I composition. Quantitative alterations of lipid and lipoprotein composition appear in the BB Wistar rat when compared to the Wistar rat, but some of the changes are more pronounced in the spontaneously diabetic BB Wistar rat.
研究了自发性糖尿病BB Wistar大鼠、非糖尿病同窝仔鼠和对照Wistar大鼠的血清脂质和脂蛋白组成，以阐明与糖尿病相关的脂蛋白组成异常。胰岛素治疗的自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的血清总甘油三酯和前β-脂蛋白浓度显著高于对照Wistar大鼠。自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的血清胆固醇和高密度脂蛋白胆固醇浓度与对照组无差异。自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）浓度高于正常大鼠。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳观察到，与对照Wistar大鼠相比，自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的VLDL中载脂蛋白E相对于总载脂蛋白C的百分比更高。通过等电聚焦，自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的VLDL和HDL中的载脂蛋白C-II相对百分比高于对照组VLDL和HDL中的载脂蛋白C-II比例。对照大鼠HDL的载脂蛋白A-I显示出四种异构体，聚焦在pI 5.8（17.3%）、5.75（30.6%）、5.65（31.8%）和5.55（20.5%）；然而，自发性糖尿病BB大鼠和非糖尿病同窝仔鼠的HDL载脂蛋白A-I主要由聚焦在pI 5.8和5.75的两种异构体代表。与对照Wistar大鼠的对应物相比，糖尿病和非糖尿病BB大鼠的VLDL都含有更高水平的酸性载脂蛋白E异构体。虽然自发性糖尿病BB大鼠的HDL胆固醇浓度保持正常，但蛋白质浓度更高，导致HDL中的胆固醇/蛋白质比率较低，这表明自发性糖尿病BB大鼠HDL的胆固醇携带能力可能低于正常水平，可能是由于载脂蛋白A-I组成异常。与Wistar大鼠相比，BB Wistar大鼠出现了脂质和脂蛋白组成的定量变化，但其中一些变化在自发性糖尿病BB Wistar大鼠中更为明显。
