Schmidt J, Erfle V, Pedersen F S, Rohmer H, Schetters H, Marquart K H, Luz A
J Gen Virol. 1984 Dec;65 ( Pt 12):2237-48. doi: 10.1099/0022-1317-65-12-2237.
Spontaneous osteomas in strain 101 mice, a strain which has a high incidence of benign bone tumours, harbour numerous C-type virus-like particles with pleomorphic characteristics. A cell-free extract from osteomas from two mice induced bone tumours, together with osteopetrosis and lymphomas, in newborn mice of the low incidence NMRI strain after a latent period of 12 to 15 months. When C3H embryo fibroblasts were infected with the osteoma extract, the resulting cell line produced virus (OA MuLVC) with a high titre. OA MuLVC was cloned by serial endpoint dilution and NIH 3T3 cells were productively infected. The resulting virus was named OA MuLVN. OA MuLVC and OA MuLVN also induced bone tumours, osteopetrosis and lymphomas 12 to 15 months after injection into newborn NMRI mice. The isolated virus showed typical characteristics of the murine retrovirus group. Fv-1 host range restriction assays classified the viruses as N-ecotropic and XC-positive. Tryptic p30 peptide analysis and RNase T1 fingerprint analysis of OA MuLVC and OA MuLVN indicated that OA MuLVC contains an Akv-like virus as well as additional components, whereas OA MuLVN is closely related to Akv, but not identical to it. Serological analysis of the envelope proteins using monoclonal antibodies also showed the virus to be similar, but not identical, to Akv virus.
在101品系小鼠（一种良性骨肿瘤发病率很高的品系）中，自发性骨瘤含有大量具有多形性特征的C型病毒样颗粒。从两只小鼠的骨瘤中提取的无细胞提取物，在潜伏期12至15个月后，在低发病率的NMRI品系新生小鼠中诱发了骨肿瘤、骨质石化和淋巴瘤。当用骨瘤提取物感染C3H胚胎成纤维细胞时，产生的细胞系产生了高滴度的病毒（OA MuLVC）。通过连续终点稀释法克隆了OA MuLVC，并有效地感染了NIH 3T3细胞。产生的病毒被命名为OA MuLVN。将OA MuLVC和OA MuLVN注射到新生NMRI小鼠体内12至15个月后，也诱发了骨肿瘤、骨质石化和淋巴瘤。分离出的病毒显示出鼠逆转录病毒组的典型特征。Fv-1宿主范围限制试验将这些病毒归类为N-亲嗜性和XC阳性。对OA MuLVC和OA MuLVN进行胰蛋白酶p30肽分析和核糖核酸酶T1指纹分析表明，OA MuLVC含有一种类似Akv的病毒以及其他成分，而OA MuLVN与Akv密切相关，但并不相同。使用单克隆抗体对包膜蛋白进行血清学分析也表明，该病毒与Akv病毒相似，但并不相同。
