Schmidt J, Luz A, Erfle V
Abteilung für Molekulare Zellpathologie, Gesellschaft für Strahlen- und Umweltforschung (GSF), Neuherberg/München, Federal Republic of Germany.
Leuk Res. 1988;12(5):393-403. doi: 10.1016/0145-2126(88)90058-6.
Female C57BL/6 and BALB/c mice were injected i.p. with 0.06 microCi/kg or 0.5 microCi/kg of the short-lived alpha-emitting radionuclide 224radium at 3-day intervals. Infectious N-ecotropic XC+, and xenotropic C-type retroviruses were activated in several tissues in both strains. In C57BL/6 mice the activation of ecotropic and xenotropic virus was dose-dependent as observed 4 weeks after the start of irradiation. In BALB/c mice a few animals showed activation of ecotropic virus after four weeks of irradiation. The expression of xenotropic virus was similar in irradiated mice and controls. Viral antigen, indicative for viraemia, was not detected in irradiated or control animals. Antiviral antibodies were found in both control and irradiated mice but higher titers were found in the irradiated mice. Bone tissue-derived N-tropic XC+ virus isolates were found to be non-oncogenic in newborn mice of the parental strain. In contrast, the same virus isolates induced a novel pattern of disease, such as osteopetrosis and osteomas together with malignant lymphomas in NMRI mice. The data indicate that the pattern of endogenous murine leukemia virus activation by internal alpha-irradiation is dependent on the dose rate, and on the genetics of the mouse strain.
雌性C57BL/6和BALB/c小鼠每隔3天腹腔注射0.06微居里/千克或0.5微居里/千克的短寿命α发射放射性核素224镭。在这两个品系的几种组织中，感染性N-亲嗜性XC+和异嗜性C型逆转录病毒被激活。在C57BL/6小鼠中，亲嗜性和异嗜性病毒的激活呈剂量依赖性，在照射开始4周后观察到。在BALB/c小鼠中，少数动物在照射4周后出现亲嗜性病毒激活。照射小鼠和对照小鼠中异嗜性病毒的表达相似。在照射或对照动物中未检测到指示病毒血症的病毒抗原。在对照小鼠和照射小鼠中均发现了抗病毒抗体，但照射小鼠中的抗体滴度更高。发现骨组织来源的N-亲嗜性XC+病毒分离株在亲本品系的新生小鼠中无致癌性。相反，相同的病毒分离株在NMRI小鼠中诱导了一种新的疾病模式，如骨质石化、骨瘤以及恶性淋巴瘤。数据表明，内部α照射激活内源性鼠白血病病毒的模式取决于剂量率和小鼠品系的遗传学。
