Hayakawa F, Ueda K, Murata K
J Nutr Sci Vitaminol (Tokyo). 1984 Aug;30(4):319-26. doi: 10.3177/jnsv.30.319.
To clarify the mechanism of thiamin degradation by catechol derivatives, thiamin and its analogues were incubated with caffeic acid or catechol in 1/15 M phosphate buffer pH 7.4 at 48 degrees C or 50 degrees C. Using thin-layer chromatography and high-performance liquid chromatography, several unknown intermediates of the reaction product, which may have both pyrimidine and catechol moieties, were detected.
为阐明儿茶酚衍生物降解硫胺素的机制，将硫胺素及其类似物与咖啡酸或儿茶酚在pH 7.4的1/15 M磷酸盐缓冲液中于48℃或50℃孵育。使用薄层色谱法和高效液相色谱法，检测到反应产物的几种未知中间体，这些中间体可能同时含有嘧啶和儿茶酚部分。
