未怀孕和怀孕后备母猪子宫冲洗液中的酸性磷酸酶和亮氨酸氨肽酶活性

Acid phosphatase and leucine aminopeptidase activity in the uterine flushings of non-pregnant and pregnant gilts.

作者信息

Zavy M T, Roberts R M, Bazer F W

出版信息

J Reprod Fertil. 1984 Nov;72(2):503-7. doi: 10.1530/jrf.0.0720503.

DOI:10.1530/jrf.0.0720503

Abstract

The activities of uteroferrin, measured as acid phosphatase (AP), and an aminoacylpeptidase (AA) were measured in uterine flushings collected from gilts on Days 6, 8, 10, 12, 14, 15, 16 and 18 of the oestrous cycle and pregnancy (N = 37). Changes in AP (P less than 0.05) were associated with day for both specific and total AP in non-pregnant and pregnant gilts. For pregnant and non-pregnant gilts, AP activity was greatest between Days 14 and 16 and then decreased to Day 18. The AA specific activity increased (P less than 0.01) between Days 10 and 12 of the oestrous cycle and pregnancy, but neither effects of pregnancy nor day by pregnancy status interaction were detected. The AA total activity was greater for pregnant gilts (P less than 0.01). These data suggest an inhibitory effect of oestrogens of blastocyst origin on synthesis and/or secretion of uteroferrin, but not AA.

摘要

以酸性磷酸酶（AP）测定的子宫铁蛋白活性以及一种氨酰基肽酶（AA）的活性，在发情周期和妊娠期第6、8、10、12、14、15、16和18天从后备母猪收集的子宫冲洗液中进行了测定（N = 37）。非妊娠和妊娠后备母猪中，特异性和总AP的AP变化均与天数相关（P < 0.05）。对于妊娠和非妊娠后备母猪，AP活性在第14至16天之间最高，然后在第18天下降。发情周期和妊娠期第10至12天之间，AA的比活性增加（P < 0.01），但未检测到妊娠效应或妊娠状态与天数之间的相互作用。妊娠后备母猪的AA总活性更高（P < 0.01）。这些数据表明，胚泡来源的雌激素对子宫铁蛋白的合成和/或分泌有抑制作用，但对AA没有抑制作用。