Vallet J L, Christenson R K, McGuire W J
USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA.
Biol Reprod. 1996 Nov;55(5):1172-8. doi: 10.1095/biolreprod55.5.1172.
The association between uteroferrin (UF, measured as acid phosphatase [AP] activity), retinol-binding protein (RBP), and transferrin (TF), within the intrauterine environment during the estrous cycle and pregnancy was examined. Pregnant gilts were killed on Days 7, 10, 13, 16, 19, 25, 30, 40, 50, 60, 70, 80, and 90. Cyclic gilts were killed on Days 7, 10, 13, and 16. On Days 7 through 19, each uterine horn was flushed with 20 ml Minimum Essential Medium. On Days 25 through 90, serum and allantoic fluid samples were collected from each fetus within each gilt and pooled for each gilt. Uterine flushings, allantoic fluid, and fetal serum samples were assayed for AP, RBP, and TF. Endometrium was collected from all gilts and cultured with [3H]-leucine; conditioned medium was measured for nondialyzable radioactive macromolecules, AP, and RBP. In uterine flushings, AP, RBP, and TF were low on Day 7, and then increased markedly (p < 0.01) by Day 13 in both cyclic and pregnant gilts. After log transformation of the data, AP was highly correlated with RBP (r = 0.99) and TF (r = 0.91, p < 0.01). Secretion of RBP and AP by endometrium in culture also increased (p < 0.01) during this period and was similar in cyclic and pregnant gilts, and RBP and AP secretion were highly correlated (r = 0.65, p < 0.01). Endometrial tissue did not secrete detectable amounts of TF in culture. Concentrations of all three proteins were low in allantoic fluid on Day 25, after which time they increased to Day 40, and then either stabilized to Day 70 followed by a decrease to Day 90 (AP and TF) or decreased to Day 90 (RBP). Endometrial secretion of both AP and RBP increased between Day 25 and 30. Then, AP increased further to Day 40 and did not change to Day 60, while RBP did not change from Day 30 to Day 60. Both decreased from Day 60 to 70, did not change to Day 80, and then increased again to Day 90. The association of RBP and TF concentrations with AP in various maternal and conceptus compartments during pregnancy is consistent with the hypothesis that one function of these two proteins may be to protect maternal and fetal tissues from lipid peroxidation that is a possible consequence of iron transport via endometrial secretion of UF.
研究了发情周期和妊娠期间子宫内环境中子宫运铁蛋白（UF，以酸性磷酸酶[AP]活性衡量）、视黄醇结合蛋白（RBP）和转铁蛋白（TF）之间的关联。在妊娠第7、10、13、16、19、25、30、40、50、60、70、80和90天处死妊娠母猪。在发情周期的第7、10、13和16天处死发情周期母猪。在第7至19天，用20毫升最低必需培养基冲洗每个子宫角。在第25至90天，从每头母猪体内的每个胎儿收集血清和尿囊液样本，并将每头母猪的样本合并。对子宫冲洗液、尿囊液和胎儿血清样本进行AP、RBP和TF检测。从所有母猪收集子宫内膜并用[3H]-亮氨酸培养；检测条件培养基中的非透析性放射性大分子、AP和RBP。在子宫冲洗液中，AP、RBP和TF在第7天较低，然后在发情周期和妊娠母猪中均在第13天显著增加（p<0.01）。对数据进行对数转换后，AP与RBP高度相关（r = 0.99），与TF也高度相关（r = 0.91，p<0.01）。在此期间，培养的子宫内膜分泌RBP和AP也增加（p<0.01），发情周期和妊娠母猪中的情况相似，且RBP和AP分泌高度相关（r = 0.65，p<0.01）。子宫内膜组织在培养中未分泌可检测量的TF。尿囊液中所有这三种蛋白质的浓度在第25天较低，此后在第40天增加，然后在第70天稳定下来，随后在第90天下降（AP和TF），或者在第90天下降（RBP）。AP和RBP在第25天至30天之间的子宫内膜分泌增加。然后，AP在第40天进一步增加，到第60天没有变化，而RBP从第30天到第60天没有变化。两者在第60天至70天下降，到第80天没有变化，然后在第90天再次增加。妊娠期间不同母体和胎儿组织中RBP和TF浓度与AP的关联与以下假设一致：这两种蛋白质的一个功能可能是保护母体和胎儿组织免受脂质过氧化的影响，脂质过氧化可能是通过子宫内膜分泌UF进行铁转运的结果。
