Schlesinger M, Bekesi J G
J Clin Lab Immunol. 1984 Sep;15(1):19-26.
The specificity of natural killer (NK) cells is still a matter of debate. In the present study a number of sugars were tested for their capacity to inhibit the NK activity of human peripheral blood lymphocytes (PBL) on K-562 target cells. The most pronounced inhibitory effect was exerted by 2-deoxy-D-glucose and 2-amino-D-glucose. Significant, though more variable inhibition was observed with D-fucose, D-ribose, L-rhamnose, and galactose. The inhibitory activity of the sugars was similar in NK assays carried out with target cells grown either in fetal calf serum or in normal human serum. 2-deoxy-D-glucose and 2-amino-D-glucose were shown to exert their inhibitory activity on effector rather than on target cells. They inhibited to a similar extent the NK activity of both unstimulated and interferon stimulated PBL. These 2 sugars also attenuated the antibody-dependent cell mediated cytotoxicity of PBL. The inhibitory effect of 2-deoxy-D-glucose and 2-amino-D-glucose on NK activity was not reversed by simultaneous exposure of NK cells to these inhibitors and glucose. The inhibitory effect of various sugars could result from the blocking of receptors on NK cells which recognize carbohydrate moieties on target cells. The activity of 2-deoxy-D-glucose and 2-amino-D-glucose could reflect impairment of the glycolytic pathway in the effector cells by the intracellular accumulation of phosphorylated derivatives of these inhibitors.
自然杀伤（NK）细胞的特异性仍是一个有争议的问题。在本研究中，测试了多种糖类抑制人外周血淋巴细胞（PBL）对K-562靶细胞的NK活性的能力。2-脱氧-D-葡萄糖和2-氨基-D-葡萄糖产生了最显著的抑制作用。D-岩藻糖、D-核糖、L-鼠李糖和半乳糖也观察到了显著但更具变异性的抑制作用。在用胎牛血清或正常人血清中培养的靶细胞进行的NK测定中，糖类的抑制活性相似。2-脱氧-D-葡萄糖和2-氨基-D-葡萄糖对效应细胞而非靶细胞发挥其抑制活性。它们对未刺激的和干扰素刺激的PBL的NK活性抑制程度相似。这两种糖类还减弱了PBL的抗体依赖性细胞介导的细胞毒性。同时将NK细胞暴露于这些抑制剂和葡萄糖下，2-脱氧-D-葡萄糖和2-氨基-D-葡萄糖对NK活性的抑制作用并未逆转。各种糖类的抑制作用可能是由于阻断了NK细胞上识别靶细胞上碳水化合物部分的受体。2-脱氧-D-葡萄糖和2-氨基-D-葡萄糖的活性可能反映了这些抑制剂的磷酸化衍生物在效应细胞内积累对糖酵解途径的损害。
