Dallegri F, Frumento G, Ballestrero A, Muttini P, Patrone F
J Clin Lab Immunol. 1984 Sep;15(1):35-7.
The purpose of the present study was to investigate the general conditions under which neutrophil polymorphonuclears (PMN) mediate antibody-independent cytolysis in the presence of normal human serum (NHS). Normal PMN were found to kill rabbit red blood cells (RRBC) only when cultured with 1% NHS. NHS was per se incapable of lysing RRBC. PMN from a patient with Chronic Granulomatous Disease did not destroy RRBC targets even in the presence of 1% NHS. In addition, cytotoxicity by normal PMN was significantly reduced by scavengers of oxygen metabolites. The results suggest that the target cell lysis by PMN in the presence of NHS requires a synergistic interaction between at least two mediators: serum factor(s) and oxygen metabolite(s).
本研究的目的是调查在正常人血清（NHS）存在的情况下，中性多形核白细胞（PMN）介导非抗体依赖性细胞溶解的一般条件。发现正常PMN仅在与1%NHS一起培养时才会杀死兔红细胞（RRBC）。NHS本身无法裂解RRBC。慢性肉芽肿病患者的PMN即使在存在1%NHS的情况下也不会破坏RRBC靶细胞。此外，氧代谢物清除剂可显著降低正常PMN的细胞毒性。结果表明，在NHS存在的情况下，PMN对靶细胞的裂解需要至少两种介质之间的协同相互作用：血清因子和氧代谢物。
