Dallegri F, Frumento G, Maggi A M, Ballestrero A, Muttini P, Patrone F
J Clin Lab Immunol. 1984 Feb;13(2):89-92.
Human neutrophils, incubated in the presence of zymosan-activated serum ( ZAS ), were able to damage ox red blood cells ( ORBC ) as assessed by a 4-hr 51Cr-release assay. Cells from a patient with chronic granulomatous disease were incapable of mediating ZAS -induced cytolysis. Cytotoxicity by normal neutrophils was prevented by N-ethyl-maleimide as well as by catalase, but heat-inactivated catalase and superoxide dismutase were completely ineffective. Heme enzyme inhibitors, azide and cyanide, only partially reduced the target cell lysis. The results indicate the involvement of products of the oxidative metabolism, mainly hydrogen peroxide, as cytolytic mediators. These mediators released by neutrophils may contribute to the tissue injury occurring during inflammation.
通过4小时的51铬释放试验评估，在酵母聚糖激活血清（ZAS）存在下孵育的人中性粒细胞能够损伤氧合红细胞（ORBC）。慢性肉芽肿病患者的细胞无法介导ZAS诱导的细胞溶解。正常中性粒细胞的细胞毒性可被N-乙基马来酰亚胺以及过氧化氢酶阻止，但热灭活的过氧化氢酶和超氧化物歧化酶则完全无效。血红素酶抑制剂叠氮化物和氰化物只能部分降低靶细胞的裂解。结果表明氧化代谢产物，主要是过氧化氢，作为细胞溶解介质参与其中。中性粒细胞释放的这些介质可能导致炎症期间发生的组织损伤。
