[大鼠肝脏中醛脱氢酶的亚细胞分布及特性]

[Subcellular distribution and properties of aldehyde dehydrogenase in the rat liver].

作者信息

Konoplitskaia K L, Kuz'mina G I, Kuz'menko L A

出版信息

Ukr Biokhim Zh (1978). 1984 Nov-Dec;56(6):624-8.

Abstract

The subcellular distribution and certain properties of rat liver aldehyde dehydrogenase are investigated. The enzyme is shown to be localized in fractions of mitochondria and microsomes. Optimal conditions are chosen for detecting the aldehyde dehydrogenase activity in the mentioned fractions. The enzyme of mitochondrial fraction shows the activity at low (0,03-0.05 mM; isoenzyme I) and high (5 mM; isoenzyme II) concentrations of the substrate. The seeming Km and V of aldehyde dehydrogenase from fractions of mitochondria and microsomes of rat liver are calculated, the acetaldehyde and NAD+ reaction being used as a substrate.

摘要

对大鼠肝脏醛脱氢酶的亚细胞分布及某些特性进行了研究。结果表明该酶定位于线粒体和微粒体组分中。选择了最佳条件来检测上述组分中的醛脱氢酶活性。线粒体组分的酶在底物浓度较低（0.03 - 0.05 mM；同工酶I）和较高（5 mM；同工酶II）时均显示出活性。以乙醛和NAD⁺反应作为底物，计算了大鼠肝脏线粒体和微粒体组分中醛脱氢酶的表观Km和V。